Methylene Blue Trihydrate Vs Methylene Blue
| Name | Methylene Blue Trihydrate | Methylene Blue |
| CAS NO. | 7220-79-3 | 61-73-4 |
| Purity | Purity 99% | Purity 99% |
| Structural formula | ![]() |
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| Solubility | Soluble in water, chloroform; sparingly soluble in ethanol | Soluble in water, ethanol, ethylene glycol, methyl cellosolve |
Methylene Blue Trihydrate and Methylene Blue are two similar substances, but they are not the same. We will analyze the similarities and differences between them from several aspects such as appearance, purity, and application.
From the perspective of chemical structure:
Methylene Blue is a common substance, while Methylene Blue Trihydrate is the trihydrate form of the former, and the active ingredients are the same.
From the aspect of appearance:
Both are usually in powder form, in a crystalline powder state. Methylene Blue is a dark green crystalline powder, while Methylene Blue Trihydrate is a dark blue-green crystalline powder.

In terms of the content of the powder:
Methylene Blue does not contain crystalline water. Under the same weight condition, the proportion of the effective substances may be higher. However, both can be produced into 99% pure high-purity powder during the production process.
In terms of stability:
The properties of Methylene Blue Trihydrate are more stable and are mostly used in industrial fields and experiments.
| Methylene Blue trihydrate Chemical Properties |
| Melting point | 190 °C (dec.)(lit.) |
| bulk density | 400-600kg/m3 |
| density | 0.98 g/mL at 25 °C |
| Fp | 14 °C |
| storage temp. | no restrictions. |
| solubility | Soluble in water, chloroform; sparingly soluble in ethanol |
| Colour Index | 52015 |
| form | Solid |
| pka | 2.6, 11.2(at 25℃) |
| color | Green |
| PH | 3 (20°C, 10g/L in H2O) |
| Water Solubility | 4 g/100 mL |
| Methylene Blue Chemical Properties |
| Melting point | 190 °C (dec.)(lit.) |
| density | 1.0 g/mL at 20 °C |
| refractive index | n20/D1.347 |
| Fp | 45 °C |
| storage temp. | room temp |
| solubility | Soluble in water, ethanol, ethylene glycol, methyl cellosolve |
| form | Liquid |
| Colour Index | 52015 |
| pka | 2.6, 11.2(at 25℃) |
| color | Green |
| Specific Gravity | 0.98 |
| Odor | Odorless |
| Water Solubility | 40 g/L (20 ºC) |
What Are The Similarities Between Methylene Blue Trihydrate And Methylene Blue?
Methylene blue trihydrate and methylene blue share similar spectral characteristics. In terms of application fields, they are used in cases of methemoglobinemia and various dyes, etc.

High-purity methylene blue trihydrate and methylene blue powder supplier, meeting pharmaceutical grade, industrial grade and other standards.Bulk Pricing Available.Ready to Ship.
COA
| Product name | Methylene Blue Trihydrate | Manufacture date | Jun.13.2025 |
| Batch number | SN250613 | Analysis date | Jun.14.2025 |
| Batch quantity | 500kg | Expiry date | Jun.13.2027 |
|
Items |
Standard |
Results |
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Characters |
Dark green to dark brown powder |
Qualified |
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Identification |
Meets the requirements |
Qualified |
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Appearance of solution |
Meets the requirements |
Qualified |
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Organic volatile impurities |
Meets the requirements |
Qualified |
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Heavy metals |
≤0.002% |
Qualified |
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Loss on drying |
≤18.0% |
14.3% |
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Residue on ignition |
≤0.1% |
0.03% |
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Assay |
99.0% |
99.3% |
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CONCLUSION: |
Complies to the standards |
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| Packing description: | Sealed export grade drum & double of sealed plastic bag |
| Storage: | Store in cool & dry place not freeze., keep away from strong light and heat |
| Shelf life: | 2 years when properly stored |
If you have any needs in this regard, please contact us to obtain samples and the lowest price. sinov@pharmachemm.com






